Daniel Barreda

Professor (Joint with BIOSCI), Faculty of Agricultural, Life and Environmental Sci - Ag, Food & Nutri Sci Dept

Contact

Professor (Joint with BIOSCI), Faculty of Agricultural, Life and Environmental Sci - Ag, Food & Nutri Sci Dept
Email
dan.barreda@ualberta.ca
Phone
(780) 492-0375
Address
5-22B Agriculture/Forestry Centre
9011 - 116 St NW
Edmonton AB
T6G 2P5

Overview

About

DEGREE 

BSc, Microbiology and Biochemistry, University of Victoria

PhD, Physiology and Cell Biology, University of Alberta 

Postdoc, Medical Immunology, University of Pennsylvania

JOB/RESEARCH AREA 

Immunology

AWARDS

A. Teaching Awards

1. Inspirational Instructor Award - Interdepartmental Science Students’ Society (2012).

2. Nominated for the Faculty of Agricultural, Life & Environmental Sciences Faculty Teaching Award (2012).

3. University of Alberta Provost Award for Early Achievement of Excellence in Undergraduate Teaching (2011). This represents the most prestigious award in undergraduate teaching at the Assistant Professor level in the University of Alberta.

4. NACTA Teaching Award of Merit - North American Colleges and Teachers of Agriculture (2011).

5. Teacher of the Year Award (2008-2009)– Department of Agricultural, Food and Nutritional Science (AFNS).

6. Teacher of the Year Award (2007-2008)– Department of Agricultural, Food and Nutritional Science (AFNS).

B. Research Awards

7. Nominated for the Canadian Society of Zoology (CSZ) Bob Boutilier New Investigator Award for Excellence in Research (2012).

8. Research and Innovation Award - Faculty of Agricultural, Life and Environmental Sciences (2011) for outstanding research contributions by a new faculty member.

9. Nominated for the NSERC Doctoral Prize for Research Excellence in Natural Sciences and Engineering (2004).

10. Nominated for the Governor General’s Gold Medal - Most Outstanding Doctoral Student at the University of Alberta (2003).

11. Banff Inflammation Workshop Young Investigator Prize (2003).

12. Alan Birdsall Memorial Scholarship: Research in Applied Fisheries Ecology & Management (2002).

13. Andrew Stewart Memorial Graduate Prize for Research Excellence (2000).

C. Scholarships and Travel Awards

14. ICI/FOCIS International Congress of Immunology Travel Award (2004).

15. NSERC Postdoctoral Fellowship (2003).

16. International Society of Developmental and Comparative Immunology (ISDCI) Travel Bursary (2003).

17. Mary Louise Imrie Graduate Student Award (2003).

18. Department of Biological Sciences Travel Award (2003).

19. University of Alberta Dissertation Fellowship + tuition/fees (2002).

20. Fisheries and Oceans Canada (DFO) Postgraduate Scholarship (2001).

21. NSERC PGS-B Scholarship (2000).

22. Walter H. Johns Graduate Fellowship (1998; 1999; 2000; 2001).

23. NSERC PGS-A Scholarship and increased stipend and Intersession Bursary (1998).

24. Graduate Teaching Assistantship (1997).

25. Walter H. Johns Tuition Scholarship (1997).

26. Faculty of Science GTA Scholarship (1997).

27. President’s Entrance Scholarship (1992).

28. Canada Scholarship – McGill University (1992 - declined).

29. B.C. Provincial Scholarship (1992).


Research
Current Research Interests                                                                               

My laboratory is interested in the evolution of leukocyte immune defense mechanisms. Broadly, we research questions in the areas of innate immunity, phagocytosis, inflammation, host-pathogen interactions, and zoonosis. My research program includes both fundamental and applied studies in topics affecting human and veterinary medicine. I hold appointments in the Department of Biological Sciences, and the Department of Agriculture, Forestry, and Nutritional Science at the University of Alberta. Currently, we are focusing our efforts on:

1) Evolution of control mechanisms for inflammation:
Our long-term objective is to dissect the contributions of phagocytes as effectors and regulators of inflammation through evolution, and to understand the implications of these evolving roles to health and disease. We focus on the balance between pro-inflammatory and homeostatic responses at infection sites because of its relevance to host immunocompetence and disease prevention. Characterization of well-conserved pathways offers important evolutionary context for generation of the complex immune networks that now exist in higher vertebrates. Moreover, characterization of more recently developed features may help identify vulnerability gaps that allow for pathogen infiltration or which contribute to unwanted immune reactions (e.g. autoimmunity).

Representative projects:
a) Evolution of phagocyte responses to pathogens and homeostatic stimuli at infection sites
b) Contributions of a novel soluble CSF-1 receptor to innate antimicrobial responses across evolution
c) Divergent roles of macrophages and neutrophils in the induction and control of inflammation

2) Comparative immunology approaches for assessment of animal and environmental health: These correspond to cross-disciplinary projects that take advantage of academic-industry-government linkages to address relevant issues in animal and environmental health. The immunity aspect provided by my lab coupled to veterinarian support ensures that industry desirable outcomes (e.g. reduction in livestock methane reduction, increased bone strength, shifts in gut microflora composition) are not associated with negative changes in immunity and health. In other projects, our comparative models are also being used as bioindicators of environmental health (e.g. changes to fish immunocompetence following remediation of industrial effluents).

Representative projects:
a) Targeted disruption of rumen methanogen activity for reduction of greenhouse gas emissions.
b) Modulation of swine immunity and health in response to vitamin D metabolites.
c) A non-antibiotic nutritional immunomodulator to control bovine respiratory disease.
d) Safe reuse water: Assessment of the removal of chemical contaminants from reuse water using a fish biomonitoring system.

Our studies take advantage of a range of hosts that include cartilaginous and bony fish, avian and rodent animal models, as well as larger animal species that are critical to the Alberta economy. We utilize high-end techniques in flow cytometry, microscopy, cellular and molecular immunology, and protein biochemistry.

For additional information (including current postings) please refer to Barreda Lab main page at:
http://grad.biology.ualberta.ca/labs/barreda/

Publications

Fish and mammalian phagocytes differentially regulate pro-inflammatory and homeostatic responses in vivo
Author(s): Rieger AM, Konowalchuk JD, Grayfer L, Katzenback BA, Havixbeck JJ, Kiemele MD, Belosevic M, Barreda DR
Publication Date: 2012
Publication: PLoS ONE
Volume: 7
Issue: 10

Pivotal advance: Peritoneal cavity B-1 B cells have phagocytic and microbicidal capacities, and present phagocytosed antigen to CD4+ T cells
Author(s): Parra D, Rieger AM, Li J, Zhang Y-A, Randall LM, Hunter CA, Barreda DR, Sunyer JO
Publication Date: 2012
Publication: Journal of Leukocyte Biology
Volume: 91
Issue: 4
Page Numbers: 525

Antimicrobial mechanisms of fish leukocytes
Author(s): Rieger AM, Barreda DR
Publication Date: 2011
Publication: Developmental and Comparative Immunology
Volume: 35
Issue: 12
Page Numbers: 1238-1245

Broiler egg storage induces cell death and influences embryo quality
Author(s): Hamidu JA, Uddin Z, Fasenko GM, Guan LL, Barreda DR
Publication Date: 2011
Publication: Poultry Science
Volume: 90
Issue: 8
Page Numbers: 1749-1757

Modified annexin V/PI apoptosis assay for accurate assessment of cell death
Author(s): Rieger AM, Nelson KL, Konowalchuk JD, Barreda DR
Publication Date: 2011
Publication: Journal of Visualized Experiments
Issue: 50
Page Numbers: 2597

Dissociation of chicken blastoderm for examination of apoptosis & necrosis by flow cytometry
Author(s): Hamidu JA, Rieger AM, Fasenko GM, Barreda DR
Publication Date: 2010
Publication: Poultry Science
Volume: 89
Issue: 5
Page Numbers: 901-909

Macrophage activation differentially modulates particle binding, phagocytosis and downstream antimicrobial mechanisms
Author(s): Rieger AM, Hall BE, Barreda DR
Publication Date: 2010
Publication: Developmental and Comparative Immunology
Volume: 34
Issue: 11
Page Numbers: 1144

Comparative immunology-based approaches to veterinary diseases
Author(s): Barreda DR, Rieger AM, Girard NC, Clarahan SDG, Eleyinmi AF
Publication Date: 2009
Publication: Encyclopedia of Life Support Systems (EOLSS)

Development of macrophages of cyprinid fish
Author(s): Hanington PC, Tam J-Y, Katzenback BA, Hitchen SJ, Barreda DR, Belosevic M
Publication Date: 2009
Publication: Developmental and Comparative Immunology
Volume: 33
Issue: 4
Page Numbers: 411-429

Microarray analysis of differentially expressed genes from Peyer's patches of cattle orally challenged with BSE
Author(s): Khaniya B, Almeida L, Basu U, Taniguchi M, Williams JL, Barreda DR, Moore SS, Guan LL
Publication Date: 2009
Publication: Journal of Toxicology and Environmental Health, Part A
Volume: 72
Issue: 17
Page Numbers: 1008-1013

Cloning and expression analysis of goldfish (Carassius auratus L.) prominin
Author(s): Walsh JG, Barreda DR, Belosevic M
Publication Date: 2007
Publication: Fish and Shellfish Immunology
Volume: 22
Page Numbers: 308-317

A novel hematopoietic granulin induces proliferation of goldfish macrophages
Author(s): Hanington PC, Barreda DR, Belosevic M
Publication Date: 2006
Publication: Journal of Biological Chemistry 14
Volume: 281
Issue: 15
Page Numbers: 9963-9970

B-lymphocytes from early vertebrates have potent phagocytic and microbicidal abilities
Author(s): Li J., Barreda DR, Zhang YA, Boshra H, Gelman AE, Lapatra S, Tort L, Sunyer JO
Publication Date: 2006
Publication: Nature Immunology
Volume: 7
Issue: 10
Page Numbers: 1116-1124

Development of goldfish macrophages in vitro
Author(s): Belosevic M, Hanington PC, and Barreda DR
Publication Date: 2006
Publication: Fish and Shellfish Immunology
Volume: 20
Issue: 2
Page Numbers: 152-171

Differential role for SRTK, Syk, and PI3 kinase in human and mouse Fc-gamma receptor phagocytosis and endocytosis
Author(s): Huang Z-Y, Barreda DR, Worth R.G., Kim M-K, Chien P., Indik Z.K., Schreiber A.D.
Publication Date: 2006
Publication: Journal of Leukocyte Biology
Volume: 80
Issue: 6
Page Numbers: 1553-1562

A novel soluble form of the CSF-1 receptor inhibits proliferation of self-renewing fish macrophages
Author(s): Barreda DR, Hanington PC, Stafford JL, Belosevic M
Publication Date: 2005
Publication: Developmental and Comparative Immunology
Volume: 29
Issue: 10
Page Numbers: 879-894

Differentially expressed genes that encode potential markers of goldfish macrophage development in vitro
Author(s): reda DR, Hanington PC, Walsh CK, Wong P, Belosevic, M
Publication Date: 2004
Publication: Developmental and Comparative Immunology
Volume: 28
Issue: 7-8
Page Numbers: 727-746

Regulation of myeloid development and function by colony stimulating factors
Author(s): Barreda DR, Hanington PC, Belosevic M
Publication Date: 2004
Publication: Developmental and Comparative Immunology
Volume: 28
Issue: 5
Page Numbers: 509-554

ATP synthase: structural changes that couple ΔμH+ to ATP synthesis
Author(s): Barreda DR
Publication Date: 2002
Publication: Crossing Boundaries (eJournal of the Graduate Students' Association, University of Alberta)
Volume: 1
Issue: 2
Page Numbers: 99-111

Antimicrobial mechanisms of fish phagocytes and their role in host defense
Author(s): Neumann NF, Stafford JL, Barreda DR, Ainsworth AJ, Belosevic M
Publication Date: 2001
Publication: Developmental and Comparative Immunology
Volume: 25
Issue: 8-9
Page Numbers: 807-825

Characterization of growth enhancing factor production in different phases of in vitro fish macrophage development
Author(s): Barreda DR, Belosevic M
Publication Date: 2001
Publication: Fish and Shellfish Immunology
Volume: 11
Page Numbers: 169-186

Transcriptional regulation of hematopoiesis
Author(s): Barreda DR, Belosevic M
Publication Date: 2001
Publication: Developmental and Comparative Immunology
Volume: 25
Issue: 8-9
Page Numbers: 763-789.

Flow cytometric analysis of PKH26-labeled goldfish kidney-derived macrophages
Author(s): Barreda DR, Neumann NF, Belosevic M
Publication Date: 2000
Publication: Developmental and Comparative Immunology
Volume: 24
Issue: 4
Page Numbers: 395-406

Generation and functional analysis of distinct macrophage sub-populations from goldfish (Carassius auratus L.) kidney leukocyte cultures
Author(s): Neumann NF, Barreda DR, Belosevic M
Publication Date: 2000
Publication: Fish and Shellfish Immunology
Volume: 10
Issue: 1
Page Numbers: 1-20

The histidine-rich protamine from ostrich and tinamou sperm. A link between reptile and bird protamines
Author(s): Ausio J, Soley JT, Burger W, Lewis JD, Barreda D, Cheng KM
Publication Date: 1999
Publication: Biochemistry
Volume: 38
Issue: 1
Page Numbers: 180-184

Production of a macrophage growth factor(s) by a macrophage cell line and macrophages derived from goldfish kidney leukocytes
Author(s): Neumann NF, Barreda D, Belosevic M
Publication Date: 1998
Publication: Developmental and Comparative Immunology
Volume: 22
Issue: 4
Page Numbers: 417-432.

The sperm nuclear basic proteins (SNBPs) of the sponge Neofibularia nolitangere: implications for the molecular evolution of SNBPs
Author(s): Ausio J, Van Veghel MLJ, Gomez R, Barreda D
Publication Date: 1997
Publication: Journal of Molecular Evolution
Volume: 45
Page Numbers: 91-96

Conventional apoptosis assays using propidium iodide generate a significant number of false positives that prevent accurate assessment of cell death.
Author(s): Rieger AM, Hall BE, Luong le T, Schang LM, Barreda DR
Publication: Journal of Immunological Methods
Volume: 358
Issue: 1-2
Page Numbers: 81-92

Influence of parent flock age on embryonic metabolism in modern turkey strains
Author(s): Hamidu JA, Fasenko GM, Guan LL, Barreda DR, and Feddes JJR
Publication: Poultry Science
Volume: 90
Issue: 2
Page Numbers: 426-434