GR Loppnow, PhD

Contact

Faculty of Science - Chemistry
Email
glen.loppnow@ualberta.ca

Overview

About

Dr. Glen R. Loppnow was born in Wisconsin and raised in Albuquerque, New Mexico. Following a BSc in chemistry and an MSc in analytical chemistry at Rensselaer Polytechnic Institute, Troy, NY, he obtained a PhD at the University of California, Berkeley, working with Rich Mathies examining the biophysical chemistry of visual pigments. After an NIH post-doctoral fellowship with Thomas G. Spiro studying photoinduced electron transfer in porphyrins at Princeton University, Dr. Loppnow headed to Canada to start his independent career in the Department of Chemistry at the University of Alberta. At Alberta, he has risen through the ranks to Professor and is currently the inaugural Associate Dean, Learning and Innovation, in the Faculty of Science. During his career, Dr. Loppnow has focused on spectroscopic characterization of excited-state structural dynamics and the resulting photochemistry in biomolecular systems, particularly nucleic acids, visual pigments and photosynthetic proteins. Lately, he has been interested in doing large-scale assays of nucleic acid damage by a large variety of insults.  He is a recipient of a 3M National Teaching Fellowship (2009), a McCalla Professorship (2009), and currently occupies a Vargo Teaching Chair (2009-present) at the University of Alberta.


Research

Photoprocesses in biology are fundamentally necessary for life and include photosynthesis, DNA damage and repair, vitamin D synthesis and vision. My research interests lie in understanding the very fast (ultrafast) dynamics in the fundamental pericyclic, and isomerization reactions underlying these bioprocesses, with nucleic acid damage in particular a recent research focus. We also develop new tools to further probe these processes and increase our understanding of them.

Our approach is to use whatever techniques are best suited for probing the environment and dynamics of biological molecules, usually spectroscopic probes, and then to develop better probes for the specific questions that arise out of these preliminary experiments. Computations are used to direct and refine hypotheses. This unique approach has allowed us to identify and quantify the structural and environmental elements which influence photochemical reactions and photophysical processes of biomolecules. This approach has also allowed us to investigate much bigger questions, such as the origins of molecular life on earth and the molecular basis for cancer. Individual projects and our progress on them are described below.

Nucleic acid excited-state dynamics and photochemistry. Currently, the largest research effort in my group is being devoted to the molecular origins and physiological consequences of the differential response of RNA and DNA to UV light and other environmental agents. While the health consequences of DNA irradiation by UV light are well-known, surprisingly little is known about RNA's response to UV light at either the molecular or cellular levels. Such studies have implications in organic photochemistry, the molecular origins of life and mechanisms for UV-induced disease, such as skin cancer and aging.  

Nucleic acid damage detection. We have worked on the development of probes to detect nucleic acid damage, including DNA and RNA molecular beacons, fluorescently labelled hairpins, fluorescent organic and inorganic dyes.  These are general, non-destructive, sensitive probes of nucleic acid damage for both in vitro and in vivo applications. The technique is more sensitive, simpler to perform, and more general in the forms of nucleic acid damage detected than current assays. We have also worked on the development of DNA and RNA microplates and microarrays for massively-parallel detection of nucleic acid damage, repair and protection on genome-wide scales. Probing deeper the structural determinants of nucleic acid reactivity provides a greater insight into the molecular factors which determine disease. By providing a platform for a complete survey of nucleic acid genome across the whole organism, causal relationships can be established in such important ailments as cancer, aging, and other DNA damage-related diseases. (Publications 59, 60) 

More details and links to papers will be uploaded soon.



Teaching

Currently, Dr. Glen R. Loppnow is teaching SCI 299, Science Citizenship.  In this science-specific take on community service learning, students work in groups and choose a global issue they'd like to work on.  Students research the science behind the global issue, write a proposal to get seed funds and implement a local solution, and present their problem, solution, and research in a creative way at the end of the year.

Glen has long believed in innovation and a diversity of delivery methods in teaching, particularly ones that include active and experiential learning.  Glen is currently the Graduate Teaching and Learning coordinator in the Department of Chemistry and holds one of the University of Alberta's Vargo Teaching Chairs.  Glen also holds a Teaching and Learning Enhancement Fund grant to look at interdisciplinary science.  With these resources, Glen has started a chemistry and science education program.

Announcements

May 4, 2015 - Welcome Chuan Du, the 2015 RISE scholar for the Loppnow Lab!  Chuan comes from UBC and just finished her second year in Chemistry.  Also, congratulations to Chuan for winning an NSERC USRA for the summer work!


May 4, 2015 - Welcome Trina Gartke to the Loppnow Lab for the summer!  Trina just finished her first year in SCI 100 at the University of Alberta and is heading towards Biochemistry.  Also, congratulations to Trina for winning the Lloyd and Margaret Cooley memorial Studentship in Analytical Chemistry! 


April 5, 2015 - Dr. Glen R. Loppnow has a current opening for 1 graduate student.


April 1, 2015 - NSERC results in and we are funded for 5 years!


March 16, 2015 - Congratulations to Sindhu Nair for passing her PhD defense!


March 12, 2015 - Congratulations to Faranak Teimoory for passing her PhD defense!

Publications

Excited-state Structural Dynamics of 6-Substituted Uracil Derivatives: Femtosecond Angle and Bond Lengthening Dynamics in Pyrimidine Nucleobase Photochemistry
Author(s): F. Teimoory, G. R. Loppnow
Publication: J. Phys. Chem. A
Volume: 118
Page Numbers: 12161-12167
External Link: http://pubs.acs.org/doi/abs/10.1021/jp510293k
Detecting UV-induced Nucleic Acid Damage
Author(s): A. F. El-Yazbi, G. R. Loppnow
Publication: Tr. Anal. Chem.
Volume: 61
Page Numbers: 83-91
External Link: http://www.sciencedirect.com/science/article/pii/S016599361400137X
Chimeric RNA-DNA Molecular Beacons for Quantification of Nucleic Acids, SNPs and Nucleic Acid Damage
Author(s): A. F. El-Yazbi, G. R. Loppnow
Publication: Anal. Chem.
Volume: 85
Page Numbers: 4321-4327
External Link: http://pubs.acs.org/doi/abs/10.1021/ac301669y
Initial Excited-State Structural Dynamics of Thymine Derivatives
Author(s): B. Billinghurst, S. A. Oladepo, G. R. Loppnow
Publication: J. Phys. Chem. B
Volume: 116
Page Numbers: 10496-10503
External Link: http://pubs.acs.org/doi/abs/10.1021/jp301952v
Multiplexed, UVC-Induced, Sequence-Dependent DNA Damage Detection
Author(s): S. G. Nair, G. R. Loppnow
Publication: Photochem. Photobiol.
Volume: 89
Page Numbers: 884-890
External Link: http://onlinelibrary.wiley.com/doi/10.1111/php.12066/pdf