John Klassen

Professor, Faculty of Science - Chemistry

Contact

Professor, Faculty of Science - Chemistry
Email
john.klassen@ualberta.ca
Phone
(780) 492-3501
Address
4-087 Centennial Ctr For Interdisciplinary SCS II
11335 Saskatchewan Drive NW
Edmonton AB
T6G 2H5

Overview

About

B.Sc., Queen's University
Ph.D., University of Alberta


Research

Our research program focuses on the development and application of mass spectrometry-based techniques, implemented with a 4.7 tesla Fourier-transform ion cyclotron resonance mass spectrometer (FT-ICR MS) equipped with a nanoflow electrospray (nanoES) source, to study the composition, topology and physicochemical properties of protein complexes in the gas and condensed phases. One component of our research program involves the use of time-resolved ion-molecule and ion-dissociation reactions to study the structure and reactivity of gaseous protein assemblies and protein-ligand complexes. These studies are of considerable fundamental importance, providing insight into the intrinsic properties of proteins and protein complexes in the absence of solvent, and are critical to the evolution of mass spectrometry as a powerful tool for proteomics. A second area of research deals with the development of novel strategies to map protein interaction sites and quantify association free energies in the aqueous phase. Some of the specific research projects in our laboratory are described below.

  1. Structural Characterization of Protein Assemblies

    We are using time-resolved dissociation experiments to dissect gaseous protein assemblies to obtain information on composition, quaternary structure and binding energetics. While the concept is straightforward, implementation is hindered by the limited fragmentation observed for the assemblies and an incomplete understanding of the dissociation mechanisms. Using the blackbody infrared radiative dissociation (BIRD) technique, we are investigating the dissociation pathways, kinetics and energetics of assemblies in the gas phase. These studies provide new insight into the dissociation mechanism and the influence of charge and higher order structure thereon and suggest new strategies to increase the structural information available from MS.

  2. Mapping Intrinsic Protein-Ligand Interactions

    Our laboratory is mapping the intermolecular interactions present in gaseous protein-ligand complexes. Using a functional group replacement strategy and a thermal activation technique, the nature and strength of the dominant intermolecular interactions in gaseous protein-oligosaccharide complexes are being determined. Comparison of the gas phase data with solution thermochemistry and crystal structures provides new insights into the structural and energetic role of solvent in association. The protein-ligand interaction energies determined in this work are also important for the development of accurate force fields for improved molecular modeling of biomolecules and their complexes.

  3. Binding Affinity and Stoichiometry of Protein-Ligand Complexes

    Another component of our research program deals with the application of nanoES-FT-ICR MS to evaluate the affinity and stoichiometry of weakly-binding protein-ligand complexes in solution. Quantification of the solution composition based on the gas-phase ion abundance is often hindered by spectral artifacts originating from the nanoES process (e.g. non-specific binding) and gas-phase processes (e.g.decomposition). We are exploring aspects of the nanoES process as well as the relative stability of specific and non-specific protein-ligand complexes to establish experimental conditions that minimize such artifacts.

Courses

CHEM 373 - Physical Properties and Dynamics of Chemical Systems

A continuation of CHEM 371 in which the physical properties of chemical systems and the dynamics and energetics of chemical processes are discussed. Topics include: colligative properties; electrochemical cells and ion activities, implications for ionic equilibria; kinetic theory and transport properties of gases and liquids; surfaces and colloid chemistry; reaction dynamics, detailed mechanisms of chemical reactions, catalysis. The emphasis will be on the development of principles of physical chemistry and their application to properties and processes of interest to chemists, biochemists, and engineers. Note: This course may not be taken for credit if credit has already been received in CHEM 273 or 275. Prerequisite: CHEM 371 or 271.

Winter Term 2021

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