Brian Rempel, PhD, BSc (Honours)

Associate Professor, Augustana - Sciences

Contact

Associate Professor, Augustana - Sciences
Address
2-308 Faith & Life Centre
4901-46 Ave
Camrose AB
T4V 2R3

Overview

About

I grew up in Edmonton, and I did my undergraduate studies at the University of Alberta (1999-2003). During my time at the UofA, I had the privilege of doing research work with three exceptional mentors:

- Dr. Ole Hindsgaul. My projects with Dr. Hindsgaul focused on measuring binding of carbohydrate ligands to lectins using Frontal Affinity Chromatography-Mass Spectrometry (FAC-MS).

- Dr. Monica Palcic. Under Dr. Palcic's supervision, I explored using Capillary Electrophoresis for single cell assays for glycosyltransferase activity, and also the expression and purification of some glycosyltransferase mutants.

- Dr. Rik Tykwinski. My work with Dr. Tykwinski was focused on measuring the stereospecificity of a series of chiral iodonium oxidants for chiral resolution of enantiomeric mixtures of alcohols.

Following my B.Sc. from the UofA, I moved to the University of British Columbia in Vancouver to work on my Ph.D. (2003-2009) under the supervision of  Dr. Stephen Withers, a world expert on the enzymes responsible for the formation and breakdown of carbohydrates. My project was on investigations of fluorinated sugars as potential diagnostic and therapeutic agents in the treatment of Gauchers disease and Mucopolysaccharidosis I, two Lysosomal Storage Diseases.

Following my Ph.D. work, I came to Augustana to teach Organic and General Chemistry, where I have been since 2009.

Besides chemistry, I enjoy spending most of my free time playing with my children. I am also an avid reader of Science Fiction and Fantasy, and I enjoy playing a variety of cardgames and boardgames which typically have a Sci-Fi/Fantasy theme to them.


Research

Research

Nature uses enzymes to speed up chemical reactions. Among the most proficient enzymes are glycosidases: enzymes responsible for the hydrolysis of a glycosidic bond to release the aglycone from the sugar residue.

I am interested in developing tools for studying glycosidase activity through the development of inhibitors, mechanistic probes, or substrates for measuring activity. One group of enzymes I am interested in are the retaining human beta-glucosidases: GBA1, GBA2, and GBA3. Deficiencies in GBA1 activity in the lysosome can lead to Gauchers disease, which is one member of a class of disorders known as Lysosomal Storage Diseases. In Gauchers disease, a buildup of unprocessed glucosylceramide substrate (which arises from the lack of GBA1 activity in the lysosome) leads to disease symptoms, through a mechanism that is only partly understood. More recently, mutations in GBA1 have also been linked to Parkinsons disease, although the connection between GBA1 and the disease is still being investigated.

To help elucidate its role in disease, it is important to be able to accurately measure GBA1 activity and expression in both cells and live organisms. Therefore, inhibitors or substrates that can report on GBA1 will be useful tools in unravelling the biochemical role this enzyme plays in both healthy and diseased individuals. This is one of the present goals of my research program. I am developing new potential substrates for GBA1 that employ self-immolative linkers to append different reporters that could be used to measure GBA1 activity with the eventual goal of preparing a PET imaging agent for GBA1 activity.

As well, in collaboration with Dr. Chris Phenix at the University of Saskatchewan, we are developing N-alkyl conduritol aziridines, a new class of covalent inactivator specific for GBA1. We recently demonstrated that these compounds are powerful inactivators of GBA1, and we are currently investigating how to develop this scaffold into a PET imaging agent for GBA1.


Teaching

Currently, the courses I teach are:
•    AUCHE 110: General Chemistry I. Offered every year in the Fall term.
•    AUCHE 112: General Chemistry II. Offered every year in the Winter term.
•    AUCHE 250: Organic Chemistry I. Offered every year in the Fall term.
•    AUCHE 252: Organic Chemistry II. Offered every year in the Winter term.
•    AUCHE 350: Organic Chemistry III. Offered in the Winter term of odd-numbered calendar years.
•    AUCHE 450: Enzymes and Enzyme Mechanisms. Offered in the Winter term of even-numbered calendar years.
•    A variety of Directed Studies and Directed Reading courses, which are individually-tailored course typically focused on my research interests. Students interested in a paper-based or lab-based research course are encouraged to contact me.
•    AUIDS 101: Topics in Liberal Arts and Sciences: Drugs. This will be my offering of the new Augustana 1st-year seminar course. My section will be focused on drugs of use and abuse, biological and chemical properties of various drugs, and the role of drugs (both beneficial and detrimental) in society. This course will be offered in the Fall block in years when scheduling permits.
•    AUIDS 137: Science Laboratory Experiences. This is a laboratory-based course that is targeted at non-Science majors. I will be responsible for the chemistry labs in this course. It will be offered in the Winter block in years when scheduling permits.


As well, I am the faculty contact for the Chemistry Demonstration project in AUCSL 361. This project involves students researching, preparing, and performing chemistry demonstrations for children attending local schools. Students interested in joining this initiative should contact me or their Academic Advisor for more details.


I am very interested in helping students develop connections between the concepts they learn in chemistry and the world around them, and my first and second-year classes make sure to include at least one such connection every day. My classes feature active learning strategies including peer-led problem solving, student-marked quizzes, and student-generated problem sets.


Rather than holding traditional office hours, students wanting  to find me are always encouraged to "Find the Nerd" in the forum with my bright pink shirt.


Courses

AUCHE 110 - General Chemistry I

A general introduction to chemistry. Topics include molecular shames and Lewis structures; states of matter and intermolecular forces; qualitative and quantitative aspects of equilibrium, acid/base chemistry and buffers. Prerequisites: Chemistry 30 and Mathematics 30-1.


AUCHE 230 - Structure and Bonding

An introduction to the principles of molecular structure and chemical bonding. Topics include: the electronic structure of atoms, bonding models and Molecular Orbital Theory, symmetry and point groups, structure and bonding of ionic solids, structure and bonding of transition metal complexes, stereochemistry and conformational isomerism. Prerequisite: AUCHE 110.


AUCHE 250 - Synthesis I

An introduction to bonding and functionality in organic compounds. Qualitative factors affecting acidity and basicity will be introduced, as well as the concept of aromaticity. A mechanistic approach to learning reactions will be emphasized, with discussion of the nucleophilic acyl additions and substitutions, nucleophilic reactions adjacent to carbonyls, simple substitutions, and electrophilic aromatic substitutions. The three-dimensional structure of molecules and the concept of stereochemistry will also be examined. Prerequisite: AUCHE 212; AUCHE 230 is recommended.


AUCHE 397 - Directed Reading I

Supervised literature research project. Prerequisite: Third-year standing. Notes: Admission to AUCHE 397 normally requires a minimum GPA of 3.0 in Chemistry. An Application for Individual Study must be completed and approved before registration in the course.


AUIDS 137 - Science Laboratory Experiences

Introduction to experimental work in Biology, chemistry, environmental science and physics. This course emphasizes numeracy, scientific communication and experimental techniques but does not presuppose any specific knowledge of disciplinary content. Note: Closed to students with *6 or more in AUBIO, AUCHE, AUENV, AUPHY and AUSCI.


AUIDS 201 - Collaborative Learning

The course allows students to learn about approaches, methodologies and/or analytic techniques specific to a discipline, while offering an opportunity to practice working collaboratively in groups on a large project. Prerequisite: AUIDS 101.


Browse more courses taught by Brian Rempel

Publications

N-Alkylated aziridines are easily-prepared, potent, specific and cell-permeable covalent inhibitors of human beta-glucocerebrosidase.

Author(s): B. T. Adams, S. Niccoli, M. A. Chowdhury, A. N. K. Esarik, S. J. Lees, B. P. Rempel, C. P. Phenix
Publication Date: 2015
Publication: Chemical Communications
Volume: 51
Issue: 57
Page Numbers: 11390-11393
External Link: http://pubs.rsc.org/en/content/articlelanding/2015/cc/c5cc03828f#!divAbstract

Carbohydrate-Modified Electrode Surfaces Sensitive to beta-Glucosidase Enzyme Activity.

Author(s): E. M. Ervin, B. T. Adams, N. Snyman, C. P. Phenix, J. K. Kariuki, B. P. Rempel
Publication Date: 2014
Publication: International Journal of Electrochemical Science
Volume: 9
Issue: 11
Page Numbers: 6043-6058
External Link: http://www.electrochemsci.org/papers/vol9/91106043.pdf

Imaging of enzyme replacement therapy using PET

Author(s): C. P. Phenix, B. P. Rempel, K. Colobong, D. J. Doudet, M. J. Adam, L. A. Clarke, S. G. Withers
Publication Date: 2010
Publication: Proceedings of the National Academy of Sciences of the United States of America
Volume: 107
Issue: 24
Page Numbers: 10842-10847
External Link: http://www.pnas.org/content/107/24/10842.long

An Improved Laboratory Synthesis of Betaine-30: A Solvatochromic Dye

Author(s): Magrieta Snyman, Brian P. Rempel
Publication: The Chemical Educator
Volume: 22
Page Numbers: 137-141
External Link: http://chemeducator.org/bibs/0022001/22170137.html

Molecular Imaging of Hydrolytic Enzymes Using PET and SPECT

Author(s): Brian P. Rempel, Eric W. Price, Christopher P. Phenix
Publication: Molecular Imaging
Volume: 16
Page Numbers: 1-30
External Link: http://journals.sagepub.com/doi/abs/10.1177/1536012117717852