John Seubert, MSc, PhD

Professor, Faculty of Pharmacy & Pharmaceutical Sciences
Adjunct Professor, Faculty of Medicine & Dentistry - Pharmacology Dept

Contact

Professor, Faculty of Pharmacy & Pharmaceutical Sciences
Email
jseubert@ualberta.ca
Phone
(780) 492-0007
Address
2020M Katz Group Centre For Research
11315 - 87 Ave NW
Edmonton AB
T6G 2H5

Adjunct Professor, Faculty of Medicine & Dentistry - Pharmacology Dept

Overview

Area of Study / Keywords

cardioprotection ischemia-reperfusion mitochondria pharmacology/toxicology fatty acids immune response cell death


About

Imagination is more important than knowledge...’ ”

― Albert Einstein


EDUCATION

BSc Biology (Emphasis Toxicology) and Minor in Psychology, 1992, Simon Fraser University, Burnaby, BC, Canada

MSc Biology - Aquatic Toxicology (Modulators of Toxicokinetics), 1997, Simon Fraser University, Burnaby, BC, Canada. Advisor: Dr. CJ Kennedy

PhD Pharmacology and Toxicology (Molecular Toxicology), 2002,  University of Western Ontario, London, Ontario, Canada. Advisor: Dr. JR Bend

PDF Cardiovascular Pharmacology, 2002-2005, National Institute of Environmental Health Sciences, RTP, NC. Advisor: Dr. DC Zeldin



Research

Cardiovascular disease (CVD) is a leading cause of morbidity and mortality in elderly individuals who have increased susceptibility to stress factors likely to cause lethal outcomes. As such, older individuals comprise greater than 75% of the patients presenting with coronary artery disease or congestive heart failure. While the etiology of age-related cardiovascular pathogenesis is poorly understood, it has been shown that deterioration of mitochondria over time plays a critical role in age-related cardiac dysfunction. Mitochondria are critical to cardiomyocyte survival and heart function, so maintenance of a healthy mitochondrial population is essential for the preservation of healthy cardiac muscle. Dietary sources of essential fatty acids have a significant effect on cardiovascular health, but many of the benefits are poorly understood.


My research focuses on investigating the role of cytochrome P450 (CYP)-derived eicosanoids in cardiovascular homeostasis and function. Specifically, we are interested in understanding the role of CYP epoxygenase metabolites of arachidonic acid (N-6 PUFA), called epoxyeicosatrieanoic acids (EETs), in maintaining tissue homeostasis and reducing injury. As well, we have recently begun exploring CYP-derived metabolites of docosahexaenoic acid (N-3 PUFA, ‘fish oil’), called epoxydocosapentaenoic acids (EDPs). Both of these 'epoxylipids', EETs and EDPs, are converted into less active diol compounds by a soluble epoxide hydrolase (sEH). We have shown that epoxylipids have significant cardioprotective properties, enabling them to limit damage to mitochondria during cardiac events. Our program is working to understand the fundamental mechanisms involved in how these epoxylipids can regulate mitochondrial quality in young and aged hearts. We are working closely with our collaborators to develop new compounds based on the structure and function of these compounds with the ultimate goal to identify therapeutic strategies that prevent and/or treat cardiac injury and improve overall cardiac health.


Major Research Questions:

1) What role do EETs and EDPs have in cardiac physiology and pathophysiology in both young and aged hearts?

2) What are the intracellular targets of EETs and EDPs?

3) How do EETs and EDPs limit mitochondrial damage from ischemic injury?

4) Can we design and develop novel compounds based on the chemical structure and function of EETs and EDPs?


PubMed Publication List





Teaching

Graduate Students – Current

Ahmed Darwesh Essa (PhD student – Jan 2017- present) Project: Improving Cardiac Mitochondria in with Novel Epoxy Lipids

Deanna Sosnowski (MSc, Sept 2019 – present) Project: Investigating Mitochondria in Cardiac Aging

Wesam Farag Bassiouni (PhD, Sept 2019 - present) Co-supervised Dr. Richard Schulz Project: Role of MMP-2 in IR Injury

Robert Valencia (MSc, Sept 2020 - Present) Project: Epoxylipids and the Innate Immune Response


Graduate Students – Past

Dharmendra Katragadda (MSc, 2009) Project: Effects of Epoxyeicosatrienoic Acid on Mitochondrial Membrane Potential

Mohamed Abukhashim (MSc, 2011) Project: Epoxyeicosatrienoic Acid Effects on GPCR Responsiveness

Sri Nagarjun Batchu (PhD, 2011) Project: Epoxyeicosatrienoic Acid Effects on Membrane Ion Channels.

Ketul Chaudhary (PhD, 2012) Project: The Role in B-Type Natriuretic Peptide in Epoxyeicosatrienoic Acid Mediated Cardioprotection  

Rawabi Qadhi (MSc, 2012) Project: The Role of N-3 PUFA and Caveolin-1 in Ischemia-Reperfusion Injury

Nasser Alsaleh (MSc, 2014) Project: Epoxyeicosatrienoic Acid Affects on Autophagy

Igor Zlobine (MSc, 2014) Project: DHA Mediated Cell Death

Jelle Vriend (Visiting MSc, 2013 – 2014) Project: Eicosanoid Regulation of LPS Mediated Cell Death

Ahmed Abdelmoneim (PhD, co-supervisor with Dr. Scot Simpson 2015) Project: Cardiovascular Risk Difference Between Glyburide and Gliclazide

Maria Akhnokh (MSc student – Jan 2013-Sept2015) Project: Epoxyeicosatrienoic Acids and Cardioprotection

Haitham El-Sikhry (PhD, 2016)  Project: Epoxyeicosatrienoic Acids and Mitochondrial Function

Tomoko Endo (visiting PhD, 2015-2018)  Project: N-3 PUFA Induced Cytotoxicity

Kristi Lockhart Jamieson (PhD, 2020) Project: Cardioprotective Effects of Soluble Epoxide Hydrolase Inhibition in Aged Hearts 

Hedieh Keshavarz-Bahaghighat (2018- ) Project: Protecting Cardiac Mitochondria with Epoxy Lipids in Aged Hearts

  

Fellows

Dr. Victor Samokhvalov (Research Associate, 2012 – 2019) Project: Mechanisms of EET-Mediated Regulation of Cell Death

Dr. Chuying Wang (Visiting Scientist, 2017- 2018) Project: Cardioprotective Mechanisms of Tetramethylpyrazine and Astragaloside IV

Dr. YunFang Zhang (Post-Doctoral Fellow, 2007 – 2009) Project: Cardioprotective Effects of EETs Toward Doxorubicin Toxicity

Dr. Fenghua Yang (Post-Doctoral Fellow, 2012 – 2013) Project: In Vivo Analysis of Mechanisms of EET-mediated Cardioprotection


Teaching

Pharmacy 203 – Introduction to Pharmacology

Pharmacy 401 – Toxicology, Drugs of Abuse and Advanced Pharmacology (new 2021)

Pharmacy 566 – Cellular Aspects of Drug Delivery and Targets 

Pharmacy 564 – Toxicology 

Pharmacy 573 – Analytical Techniques

Pharmacy 630 – Drug Metabolism

PMCOL 303 (Dept. Pharmacology) –Toxicology 



Courses

PHARM 566 - Cellular Aspects of Drug Delivery and Targets

The goals of this course are to discuss fundamental topics in cell and molecular biology and introduce students to important concepts in cellular structure and function as they relate to the design and development of novel drugs. Drug targets for macromolecules, including vaccines, proteins and genetic material will be emphasized. Prerequisite: Consent of Faculty.

Fall Term 2021

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