Arno Siraki, BSc(Hon), MSc, PhD

Professor & Director of Pharmaceutical Sciences Graduate Studies, Faculty of Pharmacy & Pharmaceutical Sciences

Contact

Professor & Director of Pharmaceutical Sciences Graduate Studies, Faculty of Pharmacy & Pharmaceutical Sciences
Email
siraki@ualberta.ca
Phone
(780) 248-1591
Address
2-020-H Katz Group Centre For Research
11315 - 87 Ave NW
Edmonton AB
T6G 2H5

Overview

About

Dr. Arno G. Siraki received his Hon.B.Sc with a specialization in toxicology in 1998 from the University of Toronto. Arno continued his graduate studies in the Department of Pharmacology (under the supervision of Dr. Peter J. O’Brien). His MSc thesis was entitled “Antioxidant and Pro-Oxidant Nature of Catecholamines“ and was conferred the degree of MSc in 2000 while working at the same time in a QC lab at GSK (then GlaxoWellcome). He selected a research path and continued graduate studies in Dr. O’Brien’s laboratory as a PhD student. He was awarded an NSERC graduate fellowship for his thesis work (“Development of quantitative structure-activity relationships for metabolic activation of drugs and xenobiotics to reactive metabolites”) and was conferred his PhD in 2004 from the Department of Pharmaceutical Sciences, Leslie Dan Faculty of Pharmacy, University of Toronto. Arno’s post-doctoral studies were carried out at the National Institute of Environmental Health Sciences (NIH), located in Research Triangle Park, NC, USA from 2004 to 2008. Under the guidance of Dr. Ronald P. Mason, Head of the Free Radical Metabolite Group, Arno developed methods to detect protein free radicals through xenobiotic metabolism. Arno was recruited to the Faculty of Pharmacy and Pharmaceutical Sciences, University of Alberta in 2008 and is currently Associate Professor. His interests involve the association of free radicals with adverse drug reactions, identification of novel electron transfer intermediates (antioxidants), and the biological role of xenobiotic-induced protein free radicals.


Research

Overaching Research Interests:

  • In general, we aim to explore drug toxicity mechanisms using various tools and techniques.
  • Investigating the biochemical mechanisms of adverse drug reactions, with particular emphasis on drugs known to cause idiosyncratic drug reactions. In my group, we aim to identify specific routes of metabolism which lead to reactive metabolites of drugs with known clinical adverse drug reactions or drug toxicity.
  • Investigating drug metabolism in leukaemia cells in order to identify compounds that can sensitize leukaemia cells to chemotherapeutic treatment.


Specific Research Interests:

  • Free radical metabolism of drugs and xenobiotics
  • Proteomic changes from free radical metabolite exposure
  • Electron transfer intermediates and novel antioxidant modulation of free radical metabolism
  • Protein radical formation and consequences
  • Neutrophil and HL-60 catalyzed free radical metabolism
  • Relationship between protein modification and apoptosis
  • Myeloperoxidase and other peroxidase enzymes involved in drugs and xenobiotic metabolism


Specialized Lab Instruments:

  • Electron paramagnetic/spin resonance spectroscopy (EPR or ESR spectroscopy). This allows our group to detect and characterize free radical intermediate through spin-trapping. Applications involve drug metabolism, beer stability/quality, membrane fluidity and others. Analysis can be performed with liquid N2 and at room temperature.
  • HPLC with diode array and charged aerosol detection.
  • Oxygen and H2O2 electrodes for biochemical or cellular studies
  • Hemavet blood analyzer - provides complete blood counts with differential for many species using < 50 uL



Teaching

Teaching Philosophy:

Teaching is a catalyst which compels inquisitive minds to pursue great questions. I recall my own experience as an undergraduate student in the toxicology program at the University of Toronto, where a fourth year lecture on oxygen toxicity ignited my curiosity and led me to pursue my career in research. One of the most important aspects of education through teaching is the connections that are made between teachers and students. This interface of learning – which can go both ways - is fundamentally important to maintaining the excellence in teaching and student achievement. 

My research program as well as my undergraduate teaching is based on an ‘open door’ approach. I do make scheduled appointments if needed, but as learning is a dynamic process, I like to “always” have time for my undergraduate and graduate students. One of the moments I appreciate most is when a student achieves a level of training such that they transition from becoming a student to a colleague. This, I feel, is one of the hallmarks of success in teaching.

Teaching areas:

  • Undergraduate: radiopharmacy and diagnostic imaging; inflammation and autoimmune disease; neuropharmacology; anti-viral pharmacology; reactive oxygen species and free radical toxicology; immunotoxicology
  • Graduate: Toxicology of pharmacuetical agents and related xenobiotics (course); toxicology of reactive metabolites

Announcements



Courses

PHARM 204 - Anatomy for Pharmacy

Provides students with a fundamental understanding of human anatomy relevant to pharmacy practice using a system-based approach. Core concepts include human anatomy and anatomical function including physiology and pathophysiology within the context of drug action. (Restricted to Pharmacy students.)

Fall Term 2021
PHARM 564 - Toxicology of Pharmaceutical Agents and Related Xenobiotics

Biochemical and molecular mechanisms of drug-induced damage. Key concepts include toxicological principles, toxicokinetics, toxic responses, bioactivation of drugs to toxic metabolites, organ directed toxicity, immunotoxicology, and receptor-mediated toxicity. Specialized topics include clinical and medical toxicology, forensic toxicology, pharmacoepidemiology related to drug toxicity, safety assessment of pharmaceutical agents, and environmental toxicology. Prerequisite: Consent of Faculty.

Fall Term 2021

Browse more courses taught by Arno Siraki

Publications

Treatment of a mixture of pharmaceuticals, herbicides and perfluorinated compounds by powdered activated carbon and ozone: Synergy, catalysis and insights into non-free OH contingent mechanisms
Author(s): Mirna Alameddine, Arno Siraki, Lusine Tonoyan, Mohamed Gamal El-Din
Publication Date: 7/10/2021
Publication: Sci Total Environ
Volume: 777
Page Numbers: 146138
External Link: https://pubmed.ncbi.nlm.nih.gov/33689896/
Interactions of the antioxidant enzymes NAD(P)H: Quinone oxidoreductase 1 (NQO1) and NRH: Quinone oxidoreductase 2 (NQO2) with pharmacological agents, endogenous biochemicals and environmental contaminants
Author(s): Md Harunur Rashid, Dinesh Babu, Arno G Siraki
Publication Date: 7/3/2021
Publication: Chem Biol Interact
Volume: 345
Page Numbers: 109574
External Link: https://pubmed.ncbi.nlm.nih.gov/34228969/
Isoniazid and host immune system interactions: A proposal for a novel comprehensive mode of action
Author(s): Saifur R Khan, Yousef Manialawy, Arno G Siraki
Publication Date: 12/1/2019
Publication: Br J Pharmacol
Volume: 176
Issue: 24
Page Numbers: 4599-4608
External Link: https://pubmed.ncbi.nlm.nih.gov/31517993/
Isoniazid and host immune system interactions: A proposal for a novel comprehensive mode of action
Author(s): Saifur R Khan, Yousef Manialawy, Arno G Siraki
Publication Date: 12/1/2019
Publication: Br J Pharmacol
Volume: 176
Issue: 24
Page Numbers: 4599-4608
External Link: https://pubmed.ncbi.nlm.nih.gov/31517993/
Myeloperoxidase-mediated oxidation of edaravone produces an apparent non-toxic free radical metabolite and modulates hydrogen peroxide-mediated cytotoxicity in HL-60 cells
Author(s): Suh, L.Y.K., Babu, D., Tonoyan, L., (...), Velázquez-Martínez, C.A., Siraki, A.G.
Publication Date: 11/1/2019
Publication: Free Radical Biology and Medicine
Volume: 143
Page Numbers: 422-432
External Link: https://www.sciencedirect.com/science/article/pii/S0891584919303715?via%3Dihub
Comprehensive in vitro characterization of PD-L1 small molecule inhibitors.
Author(s): Ganesan A, Ahmed M, Okoye I, Arutyunova E, Babu D, Turnbull WL, Kundu JK, Shields J, Agopsowicz KC, Xu L, Tabana Y, Srivastava N, Zhang G, Moon TC, Belovodskiy A, Hena M, Kandadai AS, Hosseini SN, Hitt M, Walker J, Smylie M, West FG, Siraki AG, Lemieux MJ, Elahi S, Nieman JA, Tyrrell DL, Houghton M, Barakat K.
Publication Date: 10/27/2019
Publication: Sci Rep
Volume: 9
Issue: 1
Page Numbers: 12392
External Link: https://pubmed.ncbi.nlm.nih.gov/31455818/
Caution for the routine use of phenol red – It is more than just a pH indicator
Author(s): Morgan, A., Babu, D., Reiz, B., (...), Suh, L.Y.K., Siraki, A.G.
Publication Date: 9/1/2019
Publication: Chemico-Biological Interactions
Volume: 310
Page Numbers: 108739
External Link: https://www.sciencedirect.com/science/article/pii/S0009279719307811?via%3Dihub
Comprehensive in vitro characterization of PD-L1 small molecule inhibitors
Author(s): Ganesan, A., Ahmed, M., Okoye, I., (...), Houghton, M., Barakat, K.
Publication Date: 8/27/2019
Publication: Scientific Reports
Volume: 9
Page Numbers: 12392
External Link: https://www.nature.com/articles/s41598-019-48826-6
Eosinophil peroxidase oxidizes isoniazid to form the active metabolite against M. tuberculosis, isoniazid-NAD+
Author(s): Babu, D., Morgan, A.G., Reiz, B., (...), Lacy, P., Siraki, A.G.
Publication Date: 5/1/2019
Publication: Chemico-Biological Interactions
Volume: 305
Page Numbers: 48-53
External Link: https://www.sciencedirect.com/science/article/pii/S0009279719300808?via%3Dihub