A laboratory course emphasizing introductory and advanced techniques in molecular biology. Isolation of RNA, construction of cDNA, amplification of DNA by the real time polymerase chain reaction, analysis of DNA by restriction digestion, transfection of eukaryotic cells for protein expression and Western blot analysis. Corequisite: MLSCI 480 or consent of Department. This course is designed for senior undergraduate students. Credit may only be obtained in one of MLSCI 481 or LABMP 581.