MMI 391 - Current Methods in Molecular Biology

3 units (fi 6)(FIRST, 0-0-8)

Faculty of Medicine and Dentistry

This laboratory course will introduce the student to common techniques in molecular biology. Through a series of experiments, students will clone and express the genes for bioluminescence from the light-emitting bacterium Vibrio fischeri. Technical skills will include: preparation and quantification of genomic and plasmid DNAs, screening a genomic library, restriction mapping, amplification of DNA fragments using the polymerase chain reaction, Southern blotting, expression and purification of proteins, and detection of proteins by Western blotting. Prerequisites: IMIN 200 and departmental consent. Priority given to Honors students in the IMIN program. Credit may be obtained for only one of BIOL 391, IMIN 391 or MMI 391.