Critical discussion and use of techniques for characterizing macromolecules from prokaryotic and eukaryotic systems. This course provides the theoretical and hands-on experience required to use classic and cutting-edge technologies to characterize the properties of these macromolecules. Prerequisite: consent of instructor. Lectures, assignments and exams are the same as BIOL 343 with additional assignments and evaluation appropriate to graduate studies. Credit can only be obtained for one of BIOL 343 or 543 or MICRB 343 or 345.