LABMP 581 - Techniques in Molecular Biology

3 units (fi 6)(EITHER, 1-0-5)

Faculty of Medicine and Dentistry

A laboratory course emphasizing introductory and advanced techniques in molecular biology (MLSCI 481). Isolation of RNA, construction of cDNA, amplification of DNA by the real time polymerase chain reaction, analysis of DNA by restriction digestion, transfection of eukaryotic cells for protein expression and Western blotting analysis. Prerequisites: GENET and BIOCH 200 and BIOCH 330 and consent of the Department. An oral presentation on a topic in molecular biology will be required. This course is designed for graduate students. Credit may only be obtained in one of MLSCI 481 or LABMP 581.